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KMID : 1134820080370101363
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 10 p.1363 ~ p.1368
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Extraction and Analysis of Astaxanthin from Haematococcus pluvialis Using Sonication
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Kim So-Young
Cho Eun-Ah
Yoo Ji-Min
In Man-Jin
Chae Hee-Jeong
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Abstract
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The extraction and quantitative analysis conditions for astaxanthin from Haematococcus pluvialis, and the structural characteristics of H. pluvialis extract, H. pluvialis hydrolysate and synthetic astaxanthin were investigated using UV/visible and FT-IR spectrometers. Astaxanthin was dissolved in methanol, and then treated to enhance the solubility by sonication for 45 min. With sonication pretreatment, the solubility of astaxanthin increased up to 1.5 times compared to that without sonication. The extracts were hydrolyzed by cholesterol esterase for the analysis of H. pluvialis extract containing astaxanthin ester. A HPLC method using reverse phase C18 column with methanol-water (95:5, v/v) as mobile phase was developed to analyze astaxanthin. After hydrolysis, the absorption spectrum of H. pluvialis hydrolysate was changed to similar pattern to synthetic astaxanthin, confirming the extraction and analysis condition of astaxanthin from H. pluvialis.
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KEYWORD
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astaxanthin, Haematococcus pluvialis, HPLC analysis
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